Biosafety

Vector Production Unit safety

Adeno-associated (AAV) safety

 

SECTION I – PRODUCT IDENTIFICATION
  • PRODUCT: All AAV vectors made by Vector Production Unit (UPV)
  • NAME: Recombinant adeno-associated viral vector.
  • SYNONYM OR CROSS REFERENCE: AAV vector, rAAV.
  • RISK GROUP CLASSIFICATION: Biosafety Level 1. If the transgene encodes for a potentially toxic or tumorigenic gene, then this MSDS is not applicable.
  • CHARACTERISTICS: Parvovirus; non-enveloped, 20-26 nm diameter, linear DNA genome. Replication-defective vector.
  • PROVIDED: The vector is provided as liquid or frozen particle suspensions.
  • DESCRIPTION: AAV vectors consist of recombinant transgene sequences (e.g., marker or human genes) flanked by the AAV inverted terminal repeats (ITR). ITRs are non coding regions which account for only 6% of total vector genome. Recombinant AAV vectors are produced at the UPV by the triple transfection system: (a) the ITR-containing plasmid; (b) the plasmid encoding AAV the capsid (VP1, VP2 and VP3 proteins) and replicase genes; and (c) the adenoviral helper plasmid. To remove empty capsids, at UPV AAV vectors are purified by iodixanol-based ultracentrifugation.
  • QUALITY CONTROL ASSAYS: Titer is determined by fluorescent picogreen assay (Piedra et al; Human Gene Therapy Methods 26:35–42 (2015). All vector produced must pass sterility tests to confirm absence of mycoplasm, bacteria, funghi and yeast.

 

For research use only. Not intended for human or animal diagnostic or therapeutic uses.

 

SECTION II – HEALTH HAZARDS

Handle as biohazardous material under Biosafety Level 1 containment. If the vector transgene encodes for a potentially toxic or tumorigenic gene, then this MSDS is not applicable.

  • HAZARDOUS INGREDIENTS: None.
  • CHEMICAL LISTED AS CARCINOGEN OR POTENTIAL CARCINOGEN: None.
  • FIRE AND EXPLOSION: None.
  • REACTIVITY: Stable under normal conditions. Inherently biodegradable. Does not bioaccumulate.
  • PATHOGENICITY: AAV is not known to cause any diseases in humans or animals.

 

SECTION III – SPECIAL PRECAUTIONS

The Vector Production Unit (UPV) recommends that all AAV vectors and cultures be handled by qualified biologists using appropriate safety procedures and precautions.

AAV vector genomes remain primarily episomal in target cells and have a low (if any) frequency of integration. In the presence of adenovirus (or other helper viruses) and wild-type AAV the rAAV can integrate into host cell chromosome and be shed from host. Therefore, caution should be used when using rAAV vectors in combination with helper viruses and wild-type AAV.

  • STORAGE: In sealed containers that are appropriately labeled. Long-term storage at -80°C.
  • CONTAINMENT REQUIREMENTS: Appropriate containment facilities for all activities involving the vector and vector-administered cells, tissues and fluids. This includes BSL-1 practices for rAAV vector use (including animal housing).
  • PROTECTIVE CLOTHING: Laboratory coat, gloves. Safety glasses recommended.
  • TRANSPORT INFORMATION: Not classified as dangerous in the meaning of transport regulations.

 

SECTION IV – FIRST AID/TREATMENT
  • CONTACT: Immediately flush eyes and skin with plenty of water for at least 15 minutes. Call a physician.
  • INGESTION: Wash out mouth with water. Call a physician.
  • ACCIDENTAL INJECTION: Wash area with soap and water. Call a physician.
  • ADVICE TO PHYSICIAN: No specific treatment. Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled.

 

SECTION V – METHOD OF DISPOSAL
  • SPILL: Contain spill and decontaminate the area using a disinfectant such as chlorine bleach (10% f.c.), allow sufficient contact time (30 min) before clean up.
  • WASTE DISPOSAL:
    • Dispose of viral stock by autoclaving at 121°C for 30-45 minutes. Dispose of infected liquid cultures by decontamination with chlorine bleach (10% f.c.) for 10 minutes and then dispose of in sink.
    • Dispose of infected animal carcasses or tissues by incineration.
    • Dispose of in accordance with local regulations.

 

SECTION VI – MISCELLANEOUSINFORMATION

The above information is accurate to the best of our knowledge. All materials and mixtures may present unknown hazards and should be used with caution. The Vector Production Unit (UPV) assumes no liability resulting from the handling or use of the above product.

 

Adeno-associated virus (AAV) safety sheet (.pdf)

Adenovirus safety

 

SECTION I – PRODUCT IDENTIFICATION
  • PRODUCT: All Adenovirus type-5 vectors made by Vector Production Unit (UPV).
  • NAME: Recombinant adenovirus vector type 5.
  • SYNONYM OR CROSS REFERENCE: Ad5 vector, rAd5.
  • RISK GROUP CLASSIFICATION: Biosafety Level 2. If the vector transgene encodes for a potentially toxic or tumorigenic gene, then this MSDS is not applicable.
  • CHARACTERISTICS: Adenoviridae family, non-enveloped double- stranded DNA virus linear DNA genome. The recombinant Ad5 vectors are based on human adenoviral type 5 backbone, which is deleted in the E1 and/or E3 regions. Replication-defective vector, except in permisive cell lines such as HEK293 cells.
  • PROVIDED: The vector is provided as liquid or frozen particle suspensions.
  • DESCRIPTION: Recombinant Ad5 vectors are produced at the UPV by homologous recombination and transfection in permissive human HEK293 cells. To remove empty capsides, at UPV Ad5 vectors are purified by Cesium Chloride ultracentrifugation, followed by size-exclusion chromatography.
  • QUALITY CONTROL ASSAYS: Titer in physical particles is determined by absorbance (260 nm). Titer in infectious particles is determined by measuring hexon protein in infected HEK293 cells using the AdEasy® Viral Titer Kit (cat#972500, Agilent Technologies). All vector produced must pass sterility tests to confirm absence of mycoplasm, bacteria, funghi and yeast.

 

For research use only. Not intended for human or animal diagnostic or therapeutic uses.

 

SECTION II – HEALTH HAZARDS

Handle as biohazardous material under Biosafety Level 2 containment.

  • HAZARDOUS INGREDIENTS: None.
  • CHEMICAL LISTED AS CARCINOGEN OR POTENTIAL CARCINOGEN: None.
  • FIRE AND EXPLOSION: None.
  • PATHOGENICITY: Wide clinical manisfestation and severity, including cought, conjunctivitis, pharyngitis, rhinitis and fever. Incubation Period: 1 to 10 days.

 

SECTION III – SPECIAL PRECAUTIONS

The Vector Production Unit (UPV) recommends that all Ad5 vectors and cultures be handled by qualified biologists using appropriate safety procedures and precautions.

Adenovirus does not integrate into the host cell genome but can produce a strong immune response.

  • STORAGE: In sealed containers that are appropriately labeled. Long-term storage at -80°C.
  • CONTAINMENT REQUIREMENTS: Appropriate containment facilities for all activities involving the vector and vector-administered cells, tissues and fluids. This includes BSL-2 practices for vector use (including animal housing).
  • PROTECTIVE CLOTHING: Laboratory coat, gloves and closed toed shoes. Safety glasses recommended.
  • TRANSPORT INFORMATION: Not classified as dangerous in the meaning of transport regulations.

 

SECTION IV – FIRST AID/TREATMENT 
  • CONTACT: Immediately flush eyes and skin with plenty of water for at least 15 minutes. Call a physician.
  • INGESTION: Wash out mouth with water. Call a physician.
  • ACCIDENTAL INJECTION: Wash area with soap and water. Call a physician.
  • ADVICE TO PHYSICIAN: No specific treatment. Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled.

 

SECTION V – METHOD OF DISPOSAL
  • SPILL: Contain spill and decontaminate the area using a disinfectant such as chlorine bleach (10% f.c.), allow sufficient contact time (30 min) before clean up.
  • WASTE DISPOSAL:
    • Dispose of viral stock by autoclaving at 121°C for 15 minutes. Dispose of infected liquid cultures by decontamination with chlorine bleach (10% f.c.) for 10 minutes and then dispose of in sink.
    • Dispose of infected animal carcasses or tissues by incineration.
    • Dispose of in accordance with local regulations.

 

SECTION VI – MISCELLANEOUSINFORMATION

The above information is accurate to the best of our knowledge. All materials and mixtures may present unknown hazards and should be used with caution. The Vector Production Unit (UPV) assumes no liability resulting from the handling or use of the above product.

 

Adenovirus safety sheet (.pdf)

Canine adenovirus safety

 

SECTION I – PRODUCT IDENTIFICATION
  • PRODUCT: Canine Adenovirus CAV2 vectors made by Vector Production Unit (UPV).
  • NAME: Recombinant canine adenovirus 2 vector.
  • SYNONYM OR CROSS REFERENCE: CAV2 vector, CAV2.
  • RISK GROUP CLASSIFICATION: Biosafety Level 2. If the vector transgene encodes for a potentially toxic or tumorigenic gene, then this MSDS is not applicable.
  • CHARACTERISTICS: Adenoviridae family, non-enveloped double- stranded DNA virus linear DNA genome. The recombinant CAV2 vectors are based on canine adenoviral CAV2 backbone, which is deleted in the E1 region. Replication-defective vector, except in permisive cell lines such as DKZeo cells.
  • PROVIDED: The vector is provided as liquid or frozen particle suspensions.
  • DESCRIPTION: Recombinant CAV2 vectors are produced at the UPV by homologous recombination and transfection in permissive human DKZeo cells. To remove empty capsides, at UPV CAV2 vectors are purified by Cesium Chloride ultracentrifugation, followed by size-exclusion chromatography.
  • QUALITY CONTROL ASSAYS: Titer in physical particles is determined by absorbance (260 nm). Titer in infectious particles is determined by measuring cytotoxic effect in infected DKZeo cells. All vector produced must pass sterility tests to confirm absence of mycoplasm, bacteria, funghi and yeast.

 

For research use only. Not intended for human or animal diagnostic or therapeutic uses.

 

SECTION II – HEALTH HAZARDS

Handle as biohazardous material under Biosafety Level 2 containment.

  • HAZARDOUS INGREDIENTS: None.
  • CHEMICAL LISTED AS CARCINOGEN OR POTENTIAL CARCINOGEN: None.
  • FIRE AND EXPLOSION: None.
  • PATHOGENICITY: Not associated with pathology in humans.

 

SECTION III – SPECIAL PRECAUTIONS

The Vector Production Unit (UPV) recommends that all CAV2 vectors and cultures be handled by qualified biologists using appropriate safety procedures and precautions.

Adenovirus does not integrate into the host cell genome but can produce a strong immune response.

  • STORAGE: In sealed containers that are appropriately labeled. Long-term storage at -80°C.
  • CONTAINMENT REQUIREMENTS: Appropriate containment facilities for all activities involving the vector and vector-administered cells, tissues and fluids. This includes BSL-2 practices for vector use (including animal housing).
  • PROTECTIVE CLOTHING: Laboratory coat, gloves and closed toed shoes. Safety glasses recommended.
  • TRANSPORT INFORMATION: Not classified as dangerous in the meaning of transport regulations.

 

SECTION IV – FIRST AID/TREATMENT
  • CONTACT: Immediately flush eyes and skin with plenty of water for at least 15 minutes. Call a physician.
  • INGESTION: Wash out mouth with water. Call a physician.
  • ACCIDENTAL INJECTION: Wash area with soap and water. Call a physician.
  • ADVICE TO PHYSICIAN: No specific treatment. Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled.

 

SECTION V – METHOD OF DISPOSAL
  • SPILL: Contain spill and decontaminate the area using a disinfectant such as chlorine bleach (10% f.c.), allow sufficient contact time (30 min) before clean up.
  • WASTE DISPOSAL:
    • Dispose of viral stock by autoclaving at 121°C for 15 minutes. Dispose of infected liquid cultures by decontamination with chlorine bleach (10% f.c.) for 10 minutes and then dispose of in sink.
    • Dispose of infected animal carcasses or tissues by incineration.
    • Dispose of in accordance with local regulations.

 

SECTION VI – MISCELLANEOUS INFORMATION

The above information is accurate to the best of our knowledge. All materials and mixtures may present unknown hazards and should be used with caution. The Vector Production Unit (UPV) assumes no liability resulting from the handling or use of the above product.

 

Canine adenovirus safety sheet (.pdf)

istanbul escortistanbul escortistanbul escortistanbul escortistanbul escortistanbul escortescort istanbulescort istanbulescort istanbulescort istanbul
istanbul escortistanbul escortistanbul escortistanbul escortistanbul escortistanbul escortescort istanbulescort istanbulescort istanbulescort istanbul
istanbul escortistanbul escortistanbul escortistanbul escortistanbul escortistanbul escortescort istanbulescort istanbulescort istanbulescort istanbul
porno izleporno izleporno izleporno izleporno izleporno izleporno izleporno izleporno izleporno izleporno izleporno izle